mir 33a 5p mimics Search Results


mir-33a-5p mimic  (Ribobio co)
90
Ribobio co mir-33a-5p mimic
Circ_ASAP2 binds to <t>miR-33a-5p.</t> ( A ) StarBase v3.0 online database predicted that circ_ASAP2 contained the binding sequence of miR-33a-5p. ( B and C ) Dual-luciferase reporter assay verified that circ_ASAP2 was a sponge of miR-33a-5p in both AGS and HGC-27 cells. ( D ) QRT-PCR analysis showed that the effect of circ_ASAP2 overexpression on miR-33a-5p expression in both AGS and HGC-27 cells. ( E ) QRT-PCR analysis showed that the expression level of miR-33a-5p in GES-1, AGS and HGC-27 cells and GA-induced AGS and HGC-27 cells. ( F ) QRT-PCR results determined miR-33a-5p expression in GC tissues and paracancerous normal gastric tissues. ( G ) Pearson correlation analysis showed that miR-33a-5p was negatively correlated with circ-ASAP2. * P < 0.05.
Mir 33a 5p Mimic, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mir+33a+5p+mimics/mir+33a+5p+mimic/pmc07532043-47-12-30
Average 90 stars, based on 1 article reviews
mir-33a-5p mimic - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
mir‑33a‑5p  (Shanghai GenePharma)
90
Shanghai GenePharma mir‑33a‑5p
Circ_ASAP2 binds to <t>miR-33a-5p.</t> ( A ) StarBase v3.0 online database predicted that circ_ASAP2 contained the binding sequence of miR-33a-5p. ( B and C ) Dual-luciferase reporter assay verified that circ_ASAP2 was a sponge of miR-33a-5p in both AGS and HGC-27 cells. ( D ) QRT-PCR analysis showed that the effect of circ_ASAP2 overexpression on miR-33a-5p expression in both AGS and HGC-27 cells. ( E ) QRT-PCR analysis showed that the expression level of miR-33a-5p in GES-1, AGS and HGC-27 cells and GA-induced AGS and HGC-27 cells. ( F ) QRT-PCR results determined miR-33a-5p expression in GC tissues and paracancerous normal gastric tissues. ( G ) Pearson correlation analysis showed that miR-33a-5p was negatively correlated with circ-ASAP2. * P < 0.05.
Mir‑33a‑5p, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mir+33a+5p+mimics/mir+33a+5p+mimics/pm39628827-59-4-28
Average 90 stars, based on 1 article reviews
mir‑33a‑5p - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Circ_ASAP2 binds to miR-33a-5p. ( A ) StarBase v3.0 online database predicted that circ_ASAP2 contained the binding sequence of miR-33a-5p. ( B and C ) Dual-luciferase reporter assay verified that circ_ASAP2 was a sponge of miR-33a-5p in both AGS and HGC-27 cells. ( D ) QRT-PCR analysis showed that the effect of circ_ASAP2 overexpression on miR-33a-5p expression in both AGS and HGC-27 cells. ( E ) QRT-PCR analysis showed that the expression level of miR-33a-5p in GES-1, AGS and HGC-27 cells and GA-induced AGS and HGC-27 cells. ( F ) QRT-PCR results determined miR-33a-5p expression in GC tissues and paracancerous normal gastric tissues. ( G ) Pearson correlation analysis showed that miR-33a-5p was negatively correlated with circ-ASAP2. * P < 0.05.

Journal: Cancer Management and Research

Article Title: Gambogic Acid Inhibits the Progression of Gastric Cancer via circRNA_ASAP2/miR-33a-5p/CDK7 Axis

doi: 10.2147/CMAR.S269768

Figure Lengend Snippet: Circ_ASAP2 binds to miR-33a-5p. ( A ) StarBase v3.0 online database predicted that circ_ASAP2 contained the binding sequence of miR-33a-5p. ( B and C ) Dual-luciferase reporter assay verified that circ_ASAP2 was a sponge of miR-33a-5p in both AGS and HGC-27 cells. ( D ) QRT-PCR analysis showed that the effect of circ_ASAP2 overexpression on miR-33a-5p expression in both AGS and HGC-27 cells. ( E ) QRT-PCR analysis showed that the expression level of miR-33a-5p in GES-1, AGS and HGC-27 cells and GA-induced AGS and HGC-27 cells. ( F ) QRT-PCR results determined miR-33a-5p expression in GC tissues and paracancerous normal gastric tissues. ( G ) Pearson correlation analysis showed that miR-33a-5p was negatively correlated with circ-ASAP2. * P < 0.05.

Article Snippet: Small interfering RNA against CDK7 (si-CDK7), the overexpression vector of circ_ASAP2 (circ_ASAP2), miR-33a-5p mimic (miR-33a-5p), miR-33a-5p inhibitor (anti-miR-33a-5p) and control groups, including si-NC, Vector, miR-NC, and anti-miR-NC, were purchased from Ribobio Co., Ltd. (Guangzhou, China).

Techniques: Binding Assay, Sequencing, Luciferase, Reporter Assay, Quantitative RT-PCR, Over Expression, Expressing

MiR-33a-5p blocks the effects of circ_ASAP2 overexpression on GA-mediated GC progression. ( A ) The effects between circ_ASAP2 and miR-33a-5p on miR-33a-5p expression were detected by qRT-PCR. ( B ) Colony formation assay demonstrated that the impacts among GA, circ_ASAP2 and miR-33a-5p on cell proliferation in AGS and HGC-27 cells. ( C and D ) MTT assay revealed the effects among GA, circ_ASAP2 and miR-33a-5p on cell viability in both AGS and HGC-27 cells. ( E and F ) Transwell assay showed that the effects among GA, circ_ASAP2 and miR-33a-5p on the migration and invasion of AGS and HGC-27 cells. ( G ) Flow cytometry showed that circ_ASAP2 attenuated GA-induced promotion effect on cell apoptosis in AGS and HGC-27 cells, whereas this consequence was relieved by miR-33a-5p. * P < 0.05.

Journal: Cancer Management and Research

Article Title: Gambogic Acid Inhibits the Progression of Gastric Cancer via circRNA_ASAP2/miR-33a-5p/CDK7 Axis

doi: 10.2147/CMAR.S269768

Figure Lengend Snippet: MiR-33a-5p blocks the effects of circ_ASAP2 overexpression on GA-mediated GC progression. ( A ) The effects between circ_ASAP2 and miR-33a-5p on miR-33a-5p expression were detected by qRT-PCR. ( B ) Colony formation assay demonstrated that the impacts among GA, circ_ASAP2 and miR-33a-5p on cell proliferation in AGS and HGC-27 cells. ( C and D ) MTT assay revealed the effects among GA, circ_ASAP2 and miR-33a-5p on cell viability in both AGS and HGC-27 cells. ( E and F ) Transwell assay showed that the effects among GA, circ_ASAP2 and miR-33a-5p on the migration and invasion of AGS and HGC-27 cells. ( G ) Flow cytometry showed that circ_ASAP2 attenuated GA-induced promotion effect on cell apoptosis in AGS and HGC-27 cells, whereas this consequence was relieved by miR-33a-5p. * P < 0.05.

Article Snippet: Small interfering RNA against CDK7 (si-CDK7), the overexpression vector of circ_ASAP2 (circ_ASAP2), miR-33a-5p mimic (miR-33a-5p), miR-33a-5p inhibitor (anti-miR-33a-5p) and control groups, including si-NC, Vector, miR-NC, and anti-miR-NC, were purchased from Ribobio Co., Ltd. (Guangzhou, China).

Techniques: Over Expression, Expressing, Quantitative RT-PCR, Colony Assay, MTT Assay, Transwell Assay, Migration, Flow Cytometry

Circ_ASAP2 functions as a sponge of miR-33a-5p to target CDK7 . ( A ) Starbase v3.0 online database predicted the binding sites between miR-33a-5p and CDK7 . ( B and C ) The luciferase activities were detected by dual-luciferase reporter assay in AGS and HGC-27 cells. ( D ) QRT-PCR revealed the transfection efficiency of miR-33a-5p mimics and miR-33a-5p inhibitors. ( E ) QRT-PCR evaluated CDK7 mRNA expression after miR-NC, anti-miR-NC miR-33a-5p or anti-miR-33a-5p transfection in AGS and HGC-27 cells. ( F ) The CDK7 protein expression was detected by Western blot in both AGS and HGC-27 cells. ( G and H ) The mRNA and protein levels of CDK7 were severally detected by qRT-PCR and Western blot in GES-1 cell, AGS and HGC-27 cells and GA-mediated AGS and HGC-27 cells. ( I ) The CDK7 protein expression in GC tissues was determined by Western blot. ( J ) The mRNA expression level of CDK7 in GC tissues and normal gastric tissues was assessed by qRT-PCR. ( K ) The linear relationship between miR-33a-5p and CDK7 was analyzed by Pearson correlation analysis. ( L ) The effects between circ_ASAP2 and miR-33a-5p on CDK7 mRNA expression were determined by qRT-PCR. ( M ) Western blot analysis showed the effects between circ_ASAP2 and miR-33a-5p on CDK7 protein expression. * P < 0.05.

Journal: Cancer Management and Research

Article Title: Gambogic Acid Inhibits the Progression of Gastric Cancer via circRNA_ASAP2/miR-33a-5p/CDK7 Axis

doi: 10.2147/CMAR.S269768

Figure Lengend Snippet: Circ_ASAP2 functions as a sponge of miR-33a-5p to target CDK7 . ( A ) Starbase v3.0 online database predicted the binding sites between miR-33a-5p and CDK7 . ( B and C ) The luciferase activities were detected by dual-luciferase reporter assay in AGS and HGC-27 cells. ( D ) QRT-PCR revealed the transfection efficiency of miR-33a-5p mimics and miR-33a-5p inhibitors. ( E ) QRT-PCR evaluated CDK7 mRNA expression after miR-NC, anti-miR-NC miR-33a-5p or anti-miR-33a-5p transfection in AGS and HGC-27 cells. ( F ) The CDK7 protein expression was detected by Western blot in both AGS and HGC-27 cells. ( G and H ) The mRNA and protein levels of CDK7 were severally detected by qRT-PCR and Western blot in GES-1 cell, AGS and HGC-27 cells and GA-mediated AGS and HGC-27 cells. ( I ) The CDK7 protein expression in GC tissues was determined by Western blot. ( J ) The mRNA expression level of CDK7 in GC tissues and normal gastric tissues was assessed by qRT-PCR. ( K ) The linear relationship between miR-33a-5p and CDK7 was analyzed by Pearson correlation analysis. ( L ) The effects between circ_ASAP2 and miR-33a-5p on CDK7 mRNA expression were determined by qRT-PCR. ( M ) Western blot analysis showed the effects between circ_ASAP2 and miR-33a-5p on CDK7 protein expression. * P < 0.05.

Article Snippet: Small interfering RNA against CDK7 (si-CDK7), the overexpression vector of circ_ASAP2 (circ_ASAP2), miR-33a-5p mimic (miR-33a-5p), miR-33a-5p inhibitor (anti-miR-33a-5p) and control groups, including si-NC, Vector, miR-NC, and anti-miR-NC, were purchased from Ribobio Co., Ltd. (Guangzhou, China).

Techniques: Binding Assay, Luciferase, Reporter Assay, Quantitative RT-PCR, Transfection, Expressing, Western Blot

CDK 7 silencing attenuates the effects of miR-33a-5p inhibitor on GA-induced GC progression. ( A and B ) The mRNA and protein levels of CDK7 were detected in both AGS and HGC-27 cells by qRT-PCR and Western blot, respectively. ( C ) Colony formation assay was applied to determine the effects between miR-33a-5p inhibitor and CDK7 repression on cell colony-forming ability under GA treatment in both AGS and HGC-27 cells. ( D and E ) MTT assay was employed to investigate the influences between miR-33a-5p inhibitor and CDK7 repression on cell viability after GA exposure in both AGS and HGC-27 cells. ( F and G ) The effects between miR-33a-5p inhibitor and CDK7 repression on migration and invasion of GA-induced AGS and HGC-27 cells were detected by transwell assay. ( H ) Flow cytometry analysis was carried to evaluate cell apoptosis after anti-miR-NC, anti-miR-33a-5p, anti-miR-33a-5p+si-NC or anti-miR-33a-5p+si-CDK7 transfection in both AGS and HGC-27 cells. * P < 0.05.

Journal: Cancer Management and Research

Article Title: Gambogic Acid Inhibits the Progression of Gastric Cancer via circRNA_ASAP2/miR-33a-5p/CDK7 Axis

doi: 10.2147/CMAR.S269768

Figure Lengend Snippet: CDK 7 silencing attenuates the effects of miR-33a-5p inhibitor on GA-induced GC progression. ( A and B ) The mRNA and protein levels of CDK7 were detected in both AGS and HGC-27 cells by qRT-PCR and Western blot, respectively. ( C ) Colony formation assay was applied to determine the effects between miR-33a-5p inhibitor and CDK7 repression on cell colony-forming ability under GA treatment in both AGS and HGC-27 cells. ( D and E ) MTT assay was employed to investigate the influences between miR-33a-5p inhibitor and CDK7 repression on cell viability after GA exposure in both AGS and HGC-27 cells. ( F and G ) The effects between miR-33a-5p inhibitor and CDK7 repression on migration and invasion of GA-induced AGS and HGC-27 cells were detected by transwell assay. ( H ) Flow cytometry analysis was carried to evaluate cell apoptosis after anti-miR-NC, anti-miR-33a-5p, anti-miR-33a-5p+si-NC or anti-miR-33a-5p+si-CDK7 transfection in both AGS and HGC-27 cells. * P < 0.05.

Article Snippet: Small interfering RNA against CDK7 (si-CDK7), the overexpression vector of circ_ASAP2 (circ_ASAP2), miR-33a-5p mimic (miR-33a-5p), miR-33a-5p inhibitor (anti-miR-33a-5p) and control groups, including si-NC, Vector, miR-NC, and anti-miR-NC, were purchased from Ribobio Co., Ltd. (Guangzhou, China).

Techniques: Quantitative RT-PCR, Western Blot, Colony Assay, MTT Assay, Migration, Transwell Assay, Flow Cytometry, Transfection

GA exposure decreases GC growth in vivo. ( A ) GA exposure inhibited tumor volume in vivo. ( B ) The tumor weight was decreased by GA treatment in vivo. ( C ) QRT-PCR detected circ_ASAP2 expression in GA-mediated GC tissues in vivo. ( D ) QRT-PCR determined miR-33a-5p expression in GA-mediated GC tissues in vivo. ( E and F ) The mRNA and protein levels of CDK7 were determined by qRT-PCR and Western blot, respectively, in GC tissues treated GA. * P < 0.05.

Journal: Cancer Management and Research

Article Title: Gambogic Acid Inhibits the Progression of Gastric Cancer via circRNA_ASAP2/miR-33a-5p/CDK7 Axis

doi: 10.2147/CMAR.S269768

Figure Lengend Snippet: GA exposure decreases GC growth in vivo. ( A ) GA exposure inhibited tumor volume in vivo. ( B ) The tumor weight was decreased by GA treatment in vivo. ( C ) QRT-PCR detected circ_ASAP2 expression in GA-mediated GC tissues in vivo. ( D ) QRT-PCR determined miR-33a-5p expression in GA-mediated GC tissues in vivo. ( E and F ) The mRNA and protein levels of CDK7 were determined by qRT-PCR and Western blot, respectively, in GC tissues treated GA. * P < 0.05.

Article Snippet: Small interfering RNA against CDK7 (si-CDK7), the overexpression vector of circ_ASAP2 (circ_ASAP2), miR-33a-5p mimic (miR-33a-5p), miR-33a-5p inhibitor (anti-miR-33a-5p) and control groups, including si-NC, Vector, miR-NC, and anti-miR-NC, were purchased from Ribobio Co., Ltd. (Guangzhou, China).

Techniques: In Vivo, Quantitative RT-PCR, Expressing, Western Blot